Ovine fetuses from slaughterhouses: A useful source for neural cell primary cultures

Primary cell cultures from Drosophila gastrula embryos.

Here we describe a method for preparing and culturing primary cells dissociated from Drosophila gastrula embryos. In brief, a large amount of staged embryos from young and healthy flies are collected, sterilized, and then physically dissociated into a single cell suspension using a glass homogenizer. After being plated on culture plates or chamber slides at an appropriate density in culture med...

Diffuse White Matter Injury in Ovine Fetuses

299 Received February 12, 2014; revised and accepted November 13, 2014. Published online December 12, 2014; doi: 10.1620/tjem.234.299. Correspondence: Tadashi Matsuda, M.D., Center for Perinatal-Neonatal Medicine, Tohoku University Hospital, 1-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8574, Japan. e-mail: [email protected] Cerebral Ischemia or Intrauterine Inflammation Promotes Different...

Primary Neural Stem Cell Cultures from Adult Pig Brain and Their Nerve-Regenerating Properties: Novel Strategies for Cell Therapy

Peptide-bound methionine as methionine sources for protein accretion and cell proliferation in primary cultures of ovine skeletal muscle.

Ruminants have high concentrations of peptide-bound amino acids in the circulation. Earlier studies in our laboratory showed that a myogenic cell line (C2C12) developed from mouse skeletal muscle and a mammary epithelial cell line (MAC-T) developed from bovine mammary epithelial tissues are able to utilize peptides as amino acid sources. In this study, primary cultures of ovine myogenic satelli...

Primary Dissociated Midbrain Dopamine Cell Cultures from Rodent Neonates

The ability to create primary cell cultures of dopamine neurons allows for the study of the presynaptic characteristics of dopamine neurons in isolation from systemic input from elsewhere in the brain. In our lab, we use these neurons to assess dopamine release kinetics using carbon fiber amperometry, as well as expression levels of dopamine related genes and proteins using quantitative PCR and...